GuideScan2: CRISPR Guide Design

Finds Guidescan2 vetted gRNAs for genomic regions and genes.

Organism

Enzyme

Flanking

Top N gRNAs

Filter above cutting efficiency

Filter above specificity

Filter GC-Content

Filter exonic cutting gRNAs

gRNA pattern to avoid (max 5 nucleotides)

Input genomic coordinates as chromosome:start-end, organism appropriate gene symbol, or Entrez GeneIDs. Submit one genomic coordinate per line.

Submit BED, GFF/GTF, or TXT files containing genomic coordinates.