A generalized CRISPR guideRNA design tool.

Note: Please do not run large automated jobs (i.e. using curl) against this website. Please use the downloadable version found in the Help page.

Input genomic coordinates as chromosome:start-end or organism appropriate gene symbol, one per line:


BED, GFF/GTF, Fasta, TXT file upload

TXT file contains single column of genomic coordinates in form chr#:start-end

Processing time scales with lines in file, 1000 queries takes ~5 minutes


  • Enzyme

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      Flanking: bp

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